The comparitive results of 55 and 81 serum samples from 8 and 10 flocks of goats and sheep, respectively, examined with ELISA, AGID and CFT are described. The 40 sheep sera originated from a research institute free of paratuberculosis, while all others were from well known infected flocks with paratuberculosis. The three serological methods were evaluated according to the microscopic examination and culture of fecal samples. In goats, the sensitivity and specificity of the ELISA test was 86.95% and 90.62%, the AGID was 65.21% and 84.37% and the CFT was 39.13% and 75%, respectively. In sheep the sensitivity and specificity of the ELISA test was 83.33% and 86.95%, the AGID was 61.11% and 82.6% and the CFT 55.55% and 82.60%, respectively. The present study indicates that the ELISA test is more sensitive and more specific than AGID and CFT for diagnosis of caprine and ovine paratuberculosis.