Glycolipid fractions that inhibit the killing of Candida albicans (CA) by activated bovine peripheral blood derived macrophages (Mo) were derived from M. paratuberculosis strain 18 (M avium serovar 2). Active fractions were derived utilizing the Matrix Solid Phase Dispersion technique, which is a novel method of lysis and partial fractionation of components of bacterial cells. Further fractionation of active fractions was achieved using Concanavalin A affinity chromatography and centrifugal filtration. As many as three different fractions derived from two M. paratuberculosis extracts that exhibited marked inhibition of Mo killing ability have been isolated and partially characterized. Two active fractions demonstrated characteristics typical of glycolipids and a third active fraction had characteristics compatible with a peptidoglycolipid.