| Title |
Mycobacterium avium complex isolated from swine doesn't possess IS901. |
| Author(s) |
Nishimori K,
Eguchi M,
Nakaoka Y,
Onodera Y,
Tanaka K.
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| Institution(s) |
Hokkaido Branch, Nat Inst of Anim Hlth, Hitsujigaoka-4, Sapporo, Japan, :Tokachi Livestock Hyg Serv Cent, Kawanishi, Obihiro, Japan, :Kamikawa Livestock Hyg Serv Cent, Higashitakasu, Asahikawa, Japan.
|
| Source |
Fourth International Colloquium on Paratuberculosis
|
| Section |
4:
Biology of M. avium/paratuberculosis
|
| Abstract |
The presence of mycobacterial insertion sequence IS901 in strains of mycobacteria, mainly Mycobacterium avium complex, isolated from swine was examined with PCR using IS901 specific primers (1776bp products) and primers specific for IS901 insertion sites (300bp or 1776bp products), that were descried by Kunze et. al. Four kinds of DNA extraction methods for the PCR were compared. InstaGene matrix (Bio-Rad) allowed faster and easier preparation of PCR amplifiable DNA than the NaOH-heating extraction and the SDS extraction of pretreated mycobacteria, and it provided more effective protection against the destruction of DNA than the boiling extraction. Four in 5 reference strains of serovar 1 to 3 possessed IS901. Reference strains of serovar 4, 5, 6, 8, 9, 10, 11, and 21 possessed only IS901 insertion sites. Other reference strains of serovar 7, 12, 13, 14, 15, 16, 17, 19, and 20 possessed the novel sequence (about 1070bp). All of 110 Strains of M. avium complex isolated from swine didn't possess IS901. It was suggested that no possession of IS901 was characteristic of swine-derived strains of M. avium complex.
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