Title PCR variants of Mycobacterium avium subsp. paratuberculosis.
Author(s) Nishimori K, Eguchi M, Tanaka K, Nakaoka Y1.
Institution(s) Hokkaido Branch, National Institute of Animal Health, Hitsujigaoka-4, Toyohira, Sapporo, 062; 1Tokachi Livestock Hygiene Service Center, Kisen 59-6, Kawanishi, Obihiro, 089-11, Japan.
Source Fifth International Colloquium on Paratuberculosis
Section 2: Biology of M. paratuberculosis
Abstract
Six strains of isolates from 1992 to 1995 possessed multi-PCR products (229bp and the higher molecular products), using primer 1 (139-162) and primer 2 (313-367), common IS900-detecting primer pairs. Multi-PCR products were detected using primer 1 and primer 4 (638-662), but there were only expected products, 454bp and 159pb, using primer 3 (209-233) and primer 4, alternative IS900-detecting primer pairs, and primer 2 and primer 3, respectively. It was suggested that these PCR variants possessed the homologous sequences of primer 1 upstream of the target sequence (139-367).
Source: http://www.paratuberculosis.org/pubs/proc5/page114a.htm
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