Vaccination experiments showed that vaccination against paratuberculosis is only effective in decreasing the rate of clinical disease, but it is not effective in dereasing the rate of infection itself. Regular feces culturing and culling of positive animals resulted in both vaccinated and non-vaccinated herds having a decrease of the infection rate from 7.2% at the beginning of the two year period till 1.9% at the end. A peak of excretion of Mycobacterium paratuberculosis was found in animals at the age of twelve months. Pooling of 5 feces samples showed to be a reliable method to detect positive age groups. The rate of excretion greatly influenced the least necessary time of culturing. Centrifugation of feces with a high speed centrifuge did not influence the sensitivity of the culturing significantly. Pre-cultivation with Dubos medium did not result in higher sensitivity of the culture procedure. Negative cultures in the young stock at the end of the period gives promising expectations for sanitizing these herds.