Mycobacterium paratuberculosis was first isolated in Mexico in 1975, being prevalent in ruminants from different country areas. The proliferation of this bacterium in infected animals, induces the increase immune humoral response, which is related to the amount of antigen present. It has been mentioned by other researchers, that the presence of lesions is in direct relationship with serum antibody levels. The available serological tests for the diagnosis if this disease in goats are: complement fixation (CF), agar-gel-immunodiffusion (AGID), and ELISA. These tests vary in sensitivity and specificity. The AGID test is the most recommended for the diagnosis of paratuberculosis (Ptb) in goats. The objective of this study was to compare, the AGID test with a modified commercial ELISA kit* in goats. The ELISA kit was originally designed for the diagnosis of Ptb in cattle. The following modifications were considered: higher serum samples dilution (1/350), antigoat-conjugate reagent (1/10,000), when the optical density reached 0.75 nm the reaction was stopped and modification of the time for the absorbance recording. Serum samples from 98 goats were collected in a flock from Topilejo D.F., in which the presence of the disease was already confirmed both bacteriologically and histopathologically. Seven animals had positive results to the ELISA test and 8 to the AGID test. The ELISA positive sera were also positive to AGID. The concordance between these tests was 87.5%. According to these results and the advantages that the ELISA test offers, we considered that it would be a good alternative for the diagnosis of Ptb in goats. Further studies are necessary to determine the specificity and sensitivity of the ELISA test in goats.