Title Molecular typing of Mycobacterium avium subsp. paratuberculosis based on variable numbers of tandem DNA repeats.
Author(s) Nishimori K1*, Uchida I1, Tanaka K1, Nishimori T1, Tachibana S2, Nakaoka Y2, Uemura Nobuko 2, Tamada Y2, Inahara K2, Jinma K2.
Institution(s) 1 National Institute of Animal Health, Japan. 2 Livestock Hygiene Service Centers of Hokkaido, Japan.
Source Seventh International Colloquium on Paratuberculosis
Section 1: Etiology
Abstract
Fingerprinting method based on variable numbers of tandem DNA repeats (VNTR) in 17 loci of Mycobacterium avium subsp. paratuberculosis genome was developed using BLAST search and PCR. 7 allele profiles were detected in 184 isolates. They show relatively homologous profiles compared with those of reference strains of M. avium. It was suggested that this method opened the rapid and easy way to the construction of digital databases for molecular epidemiology studies of not only M. avium subsp. paratuberculosis but also M. avium.
Source: http://www.paratuberculosis.org/pubs/proc7/abst1_p10.htm
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