| Title |
Molecular characterisation of pigmented and non-pigmented isolates of Mycobacterium avium subspecies paratuberculosis. |
| Author(s) |
Stevenson K1,
Hughes VM1*,
De Juan L2,
Inglis NF1,
Wright F3,
Sharp JM1.
|
| Institution(s) |
1 Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ Scotland, UK. 2 Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Av. Puerta de Hierro s/n, 28040 Madrid. 3 Biomathematics and Statistics Scotland, Scottish Crop Research Institute, Dundee DD2 5DA, Scotland, UK.
|
| Source |
Seventh International Colloquium on Paratuberculosis
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| Section |
1:
Etiology
|
| Abstract |
Five pigmented isolates of Mycobacterium avium subsp. paratuberculosis were examined by pulsed-field gel electrophoresis (PFGE), IS900 restriction fragment length polymorphism (IS900-RFLP) and by IS1311 polymorphism analysis using PCR. All of the pigmented isolates exhibited one of three distinct PFGE profiles with SnaB I designated 9, 10 and 11 and with Spe I designated 7, 8 and 9, which generated three multiplex profiles designated [9-7], [10-8] and [11-9]. All of the pigmented isolates had the same IS900-RFLP BstE II and Pvu II profiles. The IS900-RFLP BstE II profile was new but the IS900-RFLP Pvu II profile corresponded to Pvu II type 6 of a sheep strain described by Cousins et al. (Aust. Vet. J. 2000 78:184-190). IS1311-PCR analysis typed all of the pigmented isolates as sheep (S) strains. The genetic relationship between pigmented and non-pigmented isolates was investigated using multiplex PFGE data from the analysis of both the five pigmented isolates and 88 non-pigmented isolates of M. avium subsp. paratuberculosis from a variety of host species and geographic locations. It was possible to classify the isolates into two distinct types designated Type I, comprising the pigmented isolates, and Type II comprising the non-pigmented isolates that exhibit a very broad host range.
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