| Title |
Experimental infection of Mycobacterium avium subsp. paratuberculosis in IL-18 deficient Mice. |
| Author(s) |
Momotani E*1,
Bari ASM 1,
Aodongeril1,
Buza JJ 1,
Hikon H 1,
Tsuji N 2,
Takeda K3.
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| Institution(s) |
1 National Institute of Animal Health, 3-1-5, Kan-nondai, Tsukuba 305-0856 Japan. 2 National Institute of Agrobiological Sciences, Tsukuba, Japan. 3 Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Japan.
|
| Source |
Seventh International Colloquium on Paratuberculosis
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| Section |
2:
Pathogenesis
|
| Abstract |
To understand the role of IL-18 in the pathogenesis of paratuberculosis, we studied on experimental infected IL-18ko mice. Histo- and immunohisto-pathological changes, clinical findings, expression of inflammatory cytokines and chemokines were studied. 107 CFU of M. avium subspecies paratuberculosis (ATCC 10698) (M. paratuberculosis) were injected intraperitoneally, and examined in 1, 2, 3, 5 weeks after inoculation. Clinically, IL-18ko mice showed progressive emaciation after the infection. Hepatic epithelioid cell granulomas were formed in both groups. The number of granuloma in Ko mice was statistically higher than wild type mice. In IL-18ko mice number of the granuloma increased until 3 weeks. And then the granulomas showed maturation with large epithelioid cells. Epithelioid cell granuloma in spleen of ko mice was much severe than those in wild type mice. Granulomatous enteritis was not observed in both group in 5 weeks study, however mesenteric lymph nodes showed severe granulomatous change. Pattern of inflammatory cytokines and ckemokines were measured by RT-PCR. Role of the IL-18 in the granuloma formation and host defense will be discussed.
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