Anatomopathological lesions and the immune cellular and humoral response were evaluated in experimentally infected kids with Map obtained from goat and sheep. Twenty nine kids from a flock considered paratuberculosis free were used, divided in three groups: group A with 9 kids infected with Map from goat, group B with 9 kids infected with Map from sheep and group C with 11 kids as a control group. At 13 days post infection (dpi) 5 animals were immunizated respectively and 6 animals from the group C. The humoral and cellular response were evaluated employing Agar Gel Immuno Diffusion (AGID) and intra-dermal test (IDT) using in this last one PPDa and PPDb at different periods, anatomopathological studies were realized too. Serological results (AGID) in all groups showed similar humoral immune responses, at 29 dpi were positive diminishing at 182 dpi; positive response was stronger in the immunizated animals in comparison with non-immunizated. IDT showed similar response to both antigens (PPDa and PPDb) in immunizated animals, being stronger in comparison to infected animals in the group A and B although in the last group only was observed response to PPDb. At the anatomopathological studies focal granulomatous lesions were observed in the ileocecal valve and ileum in the animals slaughtered from the groups A and B including immunizated and no-immunizated animals being similar in severity and distribution at 120 dpi, mean while at 188 dpi lesions were more severe and extensive in jejunum. In this study the possibility of map infection in the same species and among species is evident. Also, immunization effect on development of lesions at least in early phases no showed differences in comparison with infected animals, to the contrary humoral and cellular responses were stronger in immunizated kids.