A study to compare the humoral and cellular immune responses, bacteriological and anatomopathological findings after vaccination a goat flock infected with paratuberculosis was performed. The humoral immune response in vaccinated (Group V) and non-vaccinated animals (Group NV) was temporally measured seven times by Agar Gel Immunodifussion (AGID), Enzyme-Linked Immunosorbent Assay (ELISA) and Counter Immuno-Electrophoresis (CIE). The cellular immune response was evaluated three times by delayed-type hipersensitivity skin reactions using PPD-A and PPD-B. Postmortem and bacteriologic studies were carried out in all animals that died or were culled during the study. With the 3 serological tests, Group V showed an increse in the humoral immune response between 15 and 60 days after vaccination. Group V also developed a strong a constant cellular immune response after vaccination, when compared with Group NV. However, paratuberculosis lesions in both groups were similar in severity and amount of acid-fast bacilli. Bacterial isolation was obtained was obtained from feces and tissue samples from both groups. The humoral immune response seemed to interfere with the serodiagnosis just for a brief period, while the cellular immune response was stronger and possibly would modify the course of the lesions and diminish the mycobacterial load in infected goats in a larger period of time.