Title Variable Numbers of Tandem Repeats (VNTR) Typing of Mycobacterium avium subsp. paratuberculosis Isolates in Japan
Author(s) Nishimori K1, Nakaoka Y2, Tachibana S2, Tanaka K1, Uchida I1, Nishimori T1, Saito M2, Hanyuu H2, Mori Y1, Eguchi M1.
Institution(s) 1 National Institute of Animal Health; 2 Livestock Hygiene Service Centers of Hokkaido; Japan.
Source Ninth International Colloquium on Paratuberculosis
Section 5: Epidemiology and control strategies
Presentation Poster
Abstract

Variable Numbers of Tandem Repeats (VNTR) typing is a nominee of new PCR-based molecular genotyping methods that are more rapid and discriminatory procedures than the most widely used IS900-RFLP. We have been developing the VNTR typing using 17 loci of tandem repeats for Mycobacterium avium subsp. paratuberculosis(Map). In this study, 678 strains of Map that were isolated in 203 farms (daily or beef cattle) in Japan were differentiated into 12 VNTR allele profiles that were named as Map-1 to 10, -14, and -15. Map-1 and Map-2 were 2 major profiles; 203 isolates of Map-1 (29.9%) and 361 isolates of Map-2 (53.2%) were detected in 74 farms (36.5%) and 118 farms (58.1%), respectively, but more than 2 types were detected in 20 farms. It was suggested that VNTR typing could be the first choice for molecular epidemiology of Map in Japan.
Source: http://www.paratuberculosis.org/pubs/proc9/abst150e.htm
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