Title Diagnosis of bovine paratuberculosis: sensitivity of a commercial ELISA test on bovine bulk milk
Author(s) Arrigoni N1, Cammi G1, Losini I1, Taddei R1, Tamba M2, Belletti GL1.
Institution(s) 1 Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Centro di Referenza Nazionale per la Paratubercolosi - Piacenza, Italy; 2 Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Centro Emiliano-Romagnolo Epidemiologia Veterinaria- Bologna, Italy.
Source Ninth International Colloquium on Paratuberculosis
Section 2: Diagnostic methods and quality assurance
Presentation Poster
Abstract

52 dairy herds, infected with paratuberculosis, were submitted to repeated bulk milk sampling.

183 bulk milk samples (an average of 3.5 samples/herd) were tested by a commercial ELISA test validated on milk (Institut Pourquier); 30.1% of samples and 40.4% of herds resulted reactive (positive or doubtful) to paratuberculosis. The sensitivity of this ELISA test applied on bulk milk appears strongly correlated to the herd prevalence. The repeated sampling enhances the sensitivity of this diagnostic tool.
Source: http://www.paratuberculosis.org/pubs/proc9/abst50b.htm
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