Ninth International Colloquium on Paratuberculosis

Session 2: Diagnostic methods and quality assurance

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Title Presentation Author(s)
A cultivation ring trail for Mycobacterium avium subsp. paratuberculosis in three certified laboratories. [abstract] Poster Giese SB.
Comparison of Liquid Culture to Solid Media for Quantification of Mycobacterium in Tissues Following Experimental Infection [abstract] Poster Sweeney RW, Whitlock RH, Bowersock TL, Pruitt GW.
Radiometric culture of farm slurry - inexpensive tool for the detection of paratuberculosis in dairy cattle herds [abstract] Poster Gwozdz JM, Carajias M, Mohammad I, Ridge S, Condron R.
Cross-pooled faecal culture and individual faecal PCR for paratuberculosis herd level monitoring [abstract] Poster Molina E, Sevilla I, Geijo MV, Garrido JM, Plazaola JM, Juste RA.
Culture of M. paratuberculosis from Blood [abstract] Oral Bower K, Begg D, Di Fiore L, Taylor D, Whittington R.
In vitro effect of antibiotics on Mycobacterium avium subspecies paratuberculosis in Herrold's medium [abstract] Poster Traveria GE, Pinedo MFA, Peralta LM, Bartoletti LC, Quinteros M.
Can the detection rate of faecal shedders of MAP be increased by optimising the time point of faecal sampling? [abstract] Oral Gierke F, Ziller M, Köhler HU.
Evaluation of Different Organism Based Methods for the Detection and Identification of Mycobacterium avium subspecies paratuberculosis from Bovine Feces [abstract] Oral Payeur JB, Capsel RT.
Isolation and molecular confirmation of Mycobacterium avium subsp paratuberculosis in guanacos (Lama guanicoe) in Tierra del Fuego, Chile, by fecal culture and Real-Time PCR. [abstract] Poster Salgado MÁ, Herthnek D, Bölske G, Kruze JD.
Application of culture and PCR to bulk milk for detection of paratuberculosis in dairy cattle herds [abstract] Poster Gwozdz JM, Carajias M, Mohammad I, Ridge S, Condron R.
A review of diagnostic accuracies of ELISA and faecal culture in cattle [abstract] Keynote Nielsen SS, Toft N.
Sensitivity and specificity of unique 'Multi-species indigenous ELISA kit' with respect to fecal, milk and tissues culture for the diagnosis of Johne's and Crohn's disease in India [abstract] Poster Singh SV, Singh AV, Singh PK, Sohal JS.
Variability of Repeated Johne's Disease Milk ELISA Test Results in Canadian Dairy Herds [abstract] Poster Sorge US, Kelton DF, Sears W.
An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum [abstract] Poster Marassi CD, Oelemann WM, Fonseca LS, Ristow P, Lilenbaum W.
Diagnosis of bovine paratuberculosis: sensitivity of a commercial ELISA test on bovine bulk milk [abstract] Poster Arrigoni N, Cammi G, Losini I, Taddei R, Tamba M, Belletti GL.
Evaluation of four commercial bovine-ELISA kits for the diagnosis of paratuberculosis in dairy goats [abstract] Poster Kruze JD, Salgado MÁ, Badilla XC.
Evaluation of four commercial ELISA kits for the diagnosis of bovine paratuberculosis in dairy herds of southern Chile [abstract] Poster Kruze JD, Fry MP, Salgado MÁ, Mella AF, Collins MT.
Reproducibility of results in batches of three ELISA kits for the diagnosis of paratuberculosis in cattle: recommendations for kit evaluation criteria [abstract] Poster Gwozdz JM, Carajias M.
Application of three ELISA kits to bulk milk for detection of paratuberculosis in dairy cattle herds [abstract] Poster Gwozdz JM, Carajias M, Mohammad L, Ridge S, Condron R.
Development of a novel enzyme-linked immunosorbent assay for the diagnosis of Johne's disease [abstract] Oral Eda S, Kaneko Y, Scott MC, Branscum AJ.
Development of ELISA method using purified protein derived from Mycobacterium avium subspecies Paratuberculosis isolate from Japan [abstract] Poster Wang X, Kojima H, Kato T, Ishigami S, Ishiguro S, Mori Y, Yanaka T.
Evaluation of Serological Diagnosis Capacity for Paratuberculosis Using Proficiency Testing by Interlaboratory Comparison at the Level of Veterinary Laboratories from Romania in 2006 [abstract] Poster Petriceanu G, Radulescu AR, Ragalie A, Guţu E, Tănăsuică R.
Determination of optimal conditions to test bovine milk for antibodies against Mycobacterium paratuberculosis using an ELISA test [abstract] Poster Gwozdz JM, Carajias M, Mohammad I.
Diagnostic performance of PARACHEK® for the detection of antibodies against Mycobacterium avium subsp. paratuberculosis in milk and serum of Dutch dairy cattle [abstract] Poster Weniger P, Schacher P, Senn K, Wisselink HJ, Harders-Westerveen J, Marg-Haufe B, Meissner K.
Antigenicity study of secreted proteins of Mycobacterium avium subsp paratuberculosis (Map) isolated from dairy herds in southern Chile. [abstract] Poster Pradenas MV, Jara MC, Zambrano ÁH, Kruze DJ, Collins MT.
Three-year serological follow up of an ovine herd, naturally-infected by Mycobacterium avium subsp. paratuberculosis (Map) [abstract] Poster Pourquier P, Lesceu , Foucras G.
Diagnostics for Resistance and Susceptibility to Johne's disease in deer [abstract] Poster Griffin F, Liggett S, Rodgers C.
New cases of JD and Map infection in two dairy herds from Pernambuco and Rio de Janeiro States - Brazil. [abstract] Poster Gomes MJP, Mota RA, Tokarnia CH, Galvão A, Brito MF, Seixas JN, Bercht BS, Snel GGM, Juffo GD, Chies JAB.
Diagnostic-test characteristics of microscopic examination of ZN-stained faecal smears and ELISA in cattle suspected of clinical paratuberculosis [abstract] Poster Weber MF, Verhoeff J.
The use of MPB70 and MPB83 to distinguish between bovine tuberculosis (TB) and paratuberculosis (PTB) [abstract] Poster Marassi C, McNair J, Pollock J, Ristow P, Fonseca L, Oelemann W, Lilenbaum W.
Recombinant enoyl-CoA hydratase (echA) antigen of Mycobacterium avium subspecies paratuberculosis expressed in Escherichia coli can be used for serological diagnosis of Johne's disease. [abstract] Poster Nagata R, Yoshihara K, Wang X, Yanaka T, Mori Y.
Comparative proteomic analysis of mycobacterial tuberculins and identification of Mycobacterium avium subspecies paratuberculosis antigens with diagnostic potential [abstract] Oral Santema W, Overdijk M, Barends J, Krijgsveld J, Rutten V, Koets A.
Interference of intradermal tuberculin tests on the serodiagnosis of paratuberculosis in cattle. [abstract] Oral Varges R, Marassi C, Oelemann W, Lilenbaum W.
Detection of Mycobacterium avium paratuberculosis antibodies in bovine serum using a conductometric biosensor [abstract] Oral Okafor CC, Grooms DL, Alocilja EC.
Paratuberculosis in sheep: diagnostic methods and genetic susceptibility [abstract] Poster Sezzi E, Lillini E, de Sanctis B, Scorsino G, Valentini A, Pariset L, De Grossi L.
Correlation between the occurrence of sero-doubtful and sero-positive reactions and the presence of DNA-MAP in milk. [abstract] Poster Wiszniewska A, Szteyn J, Ruszczyƒska A.
Performance of clinical and postmortem diagnostic tests during progressive stages of experimental paratuberculosis in sheep [abstract] Poster Kumar AA, Tripathi BN.
Dairy herd prevalence of Mycobacterium avium subsp. paratuberculosis in bulk-tank milk samples obtained from three regions in Fars province, Iran by nested PCR [abstract] Poster Masoud Haghkhah, Maryam Ansari Lari, Amir Mansour Novin, Ayatollah Bahrami.
Comparison of the isolation of Mycobacterium paratuberculosis from milk on the HEYM substrate and direct DNA isolation [abstract] Poster Szteyn J, Wiszniewska A, Ruszczyƒska A.
Real-time PCR testing of pooled (1:5) fecal samples comparison to HEYM culture [abstract] Keynote Whitlock RH, Mangold BL, McAdams S, Fyock T, Sweeney R, Schukken Y, Smith J, Van Kessel J, Hovingh E, Karns J, Wolfgang D, Johnson T.
Nested Polymerase Chain Reaction for the Detection of Mycobacterium avium subspecies paratuberculosis in Bovine Allantoic Fluid and Fetuses. [abstract] Oral Buergelt CD, Williams JE, Decker JH, Monif G.
'Single Colony PCR' using physical method of DNA recovery for the characterization of tiny colonies of Mycobacterium avium subspecies paratuberculosis [abstract] Poster Singh SV, Singh PK, Singh AV, Sohal JS, Subodh S, Narayanasamy K.
Comparison of four methods of DNA isolation from intestinal tissues of goats infected with Mycobacterium avium subspecies paratuberculosis and evaluation of the sensitivity of PCR with respect to tissues culture [abstract] Poster Singh PK, Singh AV, Sohal JS, Singh SV.
Ability of Simple and Nesting Polymerase Chain Reaction to Detect Mycobacterium avium subspecies paratuberculosis DNA Using Blotted Tissue Impressions of Bovine Tissue: A New Technique for Rapid Identification [abstract] Poster Williams JE, Buergelt CD, Monif GRG.
Real-time PCR for detection of Mycobacterium avium subsp. avium in milk and comparison to culture of environmental samples for herd testing [abstract] Poster Herthnek D, Nielsen SS, Bölske G.
Detection and quantification of Mycobacterium avium subsp. paratuberculosis in ovine and bovinefaeces by direct quantitative PCR [abstract] Poster Kawaji S, Taylor DL, Mori Y, Whittington RJ.
Evaluation of three methods of DNA extraction for the detection of Mycobacterium paratuberculosis by polymerase chain reaction in milk [abstract] Poster Gwozdz M, Carajias M, Bowles R.
A simple internal PCR control for Mycobacterium avium subsp. paratuberculosis constructed by PCR techniques [abstract] Poster Marsh I, McLoon M, Austin S, Fell S, Saunders V, Reddacliff L.
Results from interferon gamma testing, ELISA testing, bacteriological and pathological examination in a Norwegian goat herd with naturally acquired paratuberculosis [abstract] Oral Lybeck KR, Valheim M, Djønne B, Sølverød L, Leine N, Olsen I.
Seeing spots, developing an IFN-gamma ELISPOT assay to detect ovine M. avium subspecies paratuberculosis infection [abstract] Poster Begg D, de Silva K, Di Fiore L, Taylor D, Whittington R.
Novel diagnostic criteria of the Interferon-gamma test for bovine Paratuberculosis [abstract] Poster Mori Y, Nagata R, Yoshihara K.
Detection of Mycobacterium avium subspecies paratuberculosis by modified FASTplaqueTB bacteriophage assay [abstract] Poster De Buck J, Griffiths T, Barkema H, Rioux K.
Evaluation of fluorescence labelling of Mycobacterium avium subsp. paratuberculosis by carboxyfluorescein diacetate succinimidyl ester and carboxyfluorescein diacetate [abstract] Poster Aodongeril, Yoshihara K, Wang X, Wang H, Momotani Y, Mori Y, Momotani E.
Proteomic Analysis for Biomarkers for Johne's Disease in Sheep Serum by SELDI-TOP Mass Spectrometry [abstract] Oral Zhong L, Taylor DL, Whittington RJ.
Pathological and bacteriological diagnosis of paratuberculosis in farmed red deer (Cervus elaphus) and fallow deer (Dama dama) in Chile. [abstract] Poster Paredes EA, Pradenas MV, Kruze JD, Jara MC, Collins MT.
Pathology of ovine paratuberculosis in the North East of Portugal: A comparison of histopathology, faeces and tissue culture, polymerase chain reaction in blood, faeces and tissues, and serology [abstract] Poster Coelho AC, Pinto ML, Coelho AM, Quintas H, Pires MA, Sevilla I, Garrido JM, Rodrigues J, Juste RA.
Association of disseminated Mycobacterium avium subspecies paratuberculosis infection with severity of granulomatous enteritis and mesenteric lymphadenitis in cattle [abstract] Poster Dennis MM, Antognoli MC, Garry FB, Hirst HL, Lombard JE, Gould DH, Salman MD.
Characterization of Mycobacterium avium subsp. paratuberculosis isolates in Tamil Nadu, India. [abstract] Poster Ronald BSM, Prabhakar TG, Chandran NDJ, Edwin PG, Manohar BM.
Strain typing based on sequence polymorphisms in a surface exposed PPE protein of Mycobacterium avium subspecies paratuberculosis [abstract] Poster Griffiths T, Rioux K, De Buck J.

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